2D and 3D in vivo optical imaging platform for small animals PDF Print E-mail

From Bioluminescence to Fluorescence

This Optical imaging platform is part of a European centre of excellence that includes EMIL, Nano to Life and CLARA (Canceropôle Lyon Auvergne Rhône-Alpes)

Introduction

The small animal optical imaging platform offers a range of services that can be especially useful for lifescience organizations, notably those involved in Biomedical R&D where the need for non-invasive, spatio-temporal analyses of biological processes can be carried out directly within living organisms in real time.

Why choose the Optical Imaging Platform, Grenoble

By offering a wide range of in-vivo, optical imaging modalities (bioluminescence, 2D &3D fluorescence, macroscopiccancrologiefluorescencetomographie2.jpg fluorescence as well as per-operative fluorescence) that can be used either individually or in combination, the platform is able to provide its clients with an exceptional range of highly-targeted services.

Whether you are part of a large blue-chip company or a member of an academic team, the platform can help you drive your projects forward through its comprehensive range of services that cover a wide variety of lifescience domains including: oncology, drug development, immunology, bacteriology, virology and neurology.

Imaging of live animals under anaesthetic (gas) enables tests to be carried out on a single animal for several weeks for the same experiment thereby reducing the overall number of animals required for experimentation purposes and improving the accuracy of results.

Key benefits

  • A fast, cost-efficient alternative to "conventional techniques" that can be both difficult to implement and necessitate a large number of animals for testing
  • Access to a wide-range of cutting-edge equipment - some of which is unique on a global scale
  • A multidisciplinary approach - backed up years of highly relevant experience
  • The ability to interact with a network of private and public sector academics reputed to be leaders in their field
  • The potential to benefit from the platform's close working relationship with Grenoble's Microscopic Optical Network
  • Account management: a longterm , personalized approach to ensure that your needs are met from the beginning of your project up until the development of your brief, outlining the protocols and modalities that match your requirements.

From Bioluminescence  to 2D & 3D Fluorescence...

imagebio.jpgWhatever the imaging modality required for your research project, the Optical Imaging Platform, Grenoble, has the people and the equipment to ensure that your project runs smoothly from the development of your brief to the final analysis. The following examples demonstrate the strength in-depth of the platform and the wide range of research services that it is able to provide across different imaging platforms

Bioluminescence (Cancerology: characterization of tumor models)

  • Colour images can be developed depicting tumor and metastatic cancer growth
  • Quantification of areas of interest on the same animals over a prolonged period of time

Gene therapy: Evaluation of vectors relating to gene transfer

  • Colour images depicting reporter gene over a given period of time
  • Quantification of areas of interest on the same animals over a prolonged period of time

Regulation of gene expression:
Ongoing analysis of the regulation of gene expression in transgenic mice

  • Images illustrating reporter gene expression over a given period of time
  • Quantitative analysis relating to ≤ three animals at the same time

2D Fluorescence of the entire body (Drug Development)

Biodistribution and pharmacokinetic studies in-vivo

  • Longterm biodistribution analysis of compounds of interest (new drugs, proteins, nano particles etc)
  • In-vivo films in real time analyzing different means of injection
  • Series of images illustrating biodistribution
  • Quantification of areas of interest on animals over prolonged periods of time
  • Relative quantification of the signal obtained from the organs after dissection, as well as in plasma

Imaging at cellular levels

Macro and microscopic fluorescence

Highly-detailed 3D Fluorescence

Cancerologie : in-vivo tomographic fluorescence

  • Long term quantitative and longitudinal analysis of deep tumors (lung, brain, abdomen)
  • Long term analysis of anticancer drugs

cancrologiefluorescencetomographie.jpg

Procedural Fluorescence

  • Surgical procedures assisted by real time fluorescence imaging for:
  • Removal of lymphatic ganglions (for example sentinel ganglions)
  • Tumor removal
  • Removal of pulmonary or abdominal metastases
  • Vascular surgery

Available shortly: bi-modal imagery

Resources available

  • Cell culture room (numerous cell lines available)
  • Specially adapted animal house for nude and transgenic mice
  • Five gas anesthetic systems equipped to work between the ranges of 480nm and 690nm
  • The potential to benefit from the platform's close working relationship with Grenoble's Microscopic Optical Network

Partnerships

genetherapy.jpgInstitut Albert Bonniot - INSERM U823 : Diagnostic or therapeutic targets and vectorisation of cancer drugs : Jean-Luc Coll

Laboratoire d'Etudes Dynamiques et Structurales de la Sélectivité (Laboratory for dynamic and structural selectivity studies) UMR CNRS 5616, Pascal Dumy

Laboratoire d'imagerie et Systèmes d'Acquisition (Imaging and systems acquisition laboratory) LETI, CEA Grenoble, Philippe Rizo

Fluoptics, Grenoble
- Cyberstar, Echirolles
- Polyplus transfection, Illkirch
- NanoH, Lyon,
- Hamamatsu France

DNA Therapeutics Testimonial

"The small animal optical imaging platform has worked on several projects for our company. We are delighted to confirm that the entire team met all the requirements of our brief within the timelines that we specified. We have absolutely no hesitation in recommending your services and once again we would like to thank your for your help on this project". J.S. Sun

Contacts
Marie-Gabrielle Jouan
+33 (0)4 76 00 00 61
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TECHNOLOGY FOCUS

TESTIMONIALS

« When we identified Professor Drouet's technology at the UJF it was immediately apparent that this invention met all our needs." T Paper - Biosynex
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