Safe, fast & user-friendly high speed wholemount labelling device

Introduction

Developments in gene sequencing over the past ten years have helped us to map the human genome, resulting in data that will play a vital role in providing healthcare solutions for years to come. But to build upon this already significant body of work and take this research to the next level, a clear understanding of genetic expression must first be undertaken.

In the fields of both applied and fundamental research there is an ever-increasing need to identify where and when genes are expressed.

It is clear that the wholemount labelling process can contribute massively towards a clear understanding of how individual genes express themselves at particular times and in particular locations.

Systems have existed for some time that enable researchers to identify these markers in samples. However, up until now, a key restraint with regards to traditional whole mount labelling systems is the lead time necessary to carry out effective and large scale analyses.

Flogentec : Automatic, and fast

Flogentec's innovative new device uses a patented process to subject multiple samples to a continuous, computer controlled flow of reactants. Bespoke hardware ensures that solvents wash through 3D samples at optimum temperatures, maximizing the transfer of solvents to the specimens' core and reducing analytic lead time by over 400%. Similarly, because the device needs less highly concentrated levels of RNA probe and antibodies, research results can also be improved significantly.

Product Benefits

Whereas mRNAs are specifically targeted using the in situ hybridization "wholemount" principle, proteins are detected with specific antibodies and secondary antibodies.

The final result is a highly efficient, automatic system which can radically improve both the speed and the quality of Wholemount mapping research projects.

How it works ...

Constant flow of reactant

3D biological samples are submitted to a permanent flow of solvent which ensures that the solution is transferred to their core. A constant supply of reactant is pumped through the chamber ensuring that no stagnation occurs and that the integrity of the samples is preserved.

Recycling Process

Constant recycling of the solvent results in ARN and antibody probes that are ten times weaker than normal ensuring that background signaling is minimized and helping to reduce significantly the time that is needed to wash samples. The solvent is collected at the end of the session and can be re-used for future experiments.

Quartz flow Cells

The incubation chamber or "flow cell" is made out of quartz which helps ensure highly precise levels of thermoregulation. The chamber can hold between 10 to 20 samples. However, dependent on their size, between 40 and 300 samples can be analyzed in one experiment in 24 hours.

Ability to multi-task

Four individual experiments can be carried out simultaneously with different probes and temperatures. It is possible to test in one session, a probe with four different hybridization temperatures.

Total autonomy

The user settings for each experiment are imported into the Flogentec device via a USB port. The machine is then able to function in its own right without the need to be connected to a PC. Once the experiment has finished a summary of the session can be exported back to the PC via the USB port for further analysis.

Compact size

The Flogentec device works entirely on low voltage (24 Volts). A back up power supply (batteries) ensures that the system can continue to function following a power cut. Its compact size makes it easy to use.

The Flogentec device works entirely on low voltage (24 Volts). A back up power supply (batteries) ensures that the system can continue to function following a power cut.

Commercial Opportunities

To find out more about this innovative device, please contact us for more information.

Contact
Pascale Grenard-Ecuyer
Tel. +33(0)4 76 00 78 34
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