RNAXIS: The next generation of services in RNA analysis

Introduction

RNA is one of the most important components of all living organisms. For complex organisms it represents the first functional level of genomic expression and for certain viruses RNA is the only means of extrapolating genetic information.

However, RNA analysis is more complex than DNA analysis because RNA molecules are so much more sensitive to degradation. This means that specific competencies are required to manipulate and transform RNA into more stable single or double strand cDNA components – a factor crucial to the effective analysis of any RNA samples.

RNAXIS - A Business Unit of Floralis, the Technology Transfer Subsidiary, of the University Joseph Fourier, Grenoble - 1, is a research and development organization dedicated to providing a range of molecular biology research services centered around three core themes:

• RNA purification, cDNA synthesis and sequencing.
• RNA biomarker discovery.
• Bespoke RNA research services and project development.

Benefits

Researchers working in both the fields of public and private research can exploit the many benefits of the RNAXIS offering:

• A universal double strand cDNA synthesis method: open up endless possibilities with regards to full genome RNA virus and bacterial transcriptome sequencing.
• Robust know-how and technologies dedicated to RNA Biomarker discovery.
• Service and expertise:  access to a unique RNA analysis platform.

• Projects delivered on time & on budget

• Possibility for French private companies to access “Crédit Impôt Recherche” (a means of offsetting research budget on tax).
• Consultancy: bespoke RNA consultancy services available.

You can download our offering here

Applications

Our comprehensive range of RNA analysis services can generate important information on all of the following:

• Transcriptome
  
  • Qualitative and quantitative data about mRNA and small RNA expression.

• RNA virus genome
  
  • Full genome sequencing, virus identification and virus classification.
  • Partial genome sequencing, SNP discovery and therapeutic resistant mutation diagnosis.

Next-Gen Sequencing opens new opportunities in pathogen detection and particularly for RNA virus. Generally, extracted RNA from biological samples contains more RNA from the host than from pathogen. For improving the yield of specific sequences, RNAXIS provides a process for enriching RNA pathogen. Coupled to this enrichment process, RNAXIS also provides a Universal Amplification of RNA that produces double strand DNA fragments fully compatible with Next-Gen sequencing.

Core services

RNA purification:

• Total RNA.
• mRNA.
• small non-coding RNA.

Double strand cDNA synthesis (compatible with Next-generation sequencing):

• Eukaryote and prokaryote organisms.
• RNA virus.

Differential expression analysis:

• Microarrays (mRNA and miRNA).
• Real-time PCR.

Custom projects:

• Development of the project brief.
• Implementation of the project and subsequent analysis.
• Project delivery with full report.

You can find out more about our project in the following links :

Transcriptomic analysis

Custom project

Complete Viral genom RNA sequencing


Technical platforms