Grenoble Medical Proteomic Platform (clinical bias)
Expert in the processing and analysis of clinical assays, this leading proteomic platform capitalizes on its close proximity with Grenoble University Hospital to advance research in proteomics
Overview
Expert in a wide variety of cutting-edge proteomic techniques such as peptidic fractioning using OFF-GEL and quantification by iTRAQ the platform has also developed an excellent reputation for protein analysis of liquid biological samples (plasma for example).
The expertise in analyzing liquid biological samples is due, in no small part, to the team's close working affinity with Grenoble University Hospital. The combination of cutting-edge equipment available at the platform and its close alliance with Grenoble CHU results in a high-quality medical proteomic service that is of specific interest to private and public sector research organisations. With Grenoble as its base, the platform also has close links with nano and biotech research programmes (Nanobio).
The platform is one of the few centres in France that is able to combine two types of sources of ionization for mass spectrometry and has already managed over 15 highly complex projects (some examples can be found below).
Key Benefits
Clinical focus: the platform has been developed specifically to manage clinical research programmes
Affinity with Grenoble CHU: located at the heart of Grenoble University Hospital, the platform continues to maintain its close working relationship with the CHU, a factor that facilitates the exchange of medical/clinical data as well as enabling access to a biological resource centre.
Development of specific techniques relating to clinical samples (plasma, tissues)
Specialists in post-marker quantitative techniques (iTRAQ techniques) and multiple proteomic analyses
Unique proteomic and peptide separation processes
Industrial training sessions: the team can carry out training sessions on request
Research contracts established with the platform conform to BPL (bon pratique de laboratoire) standards. The team is currently working towards gaining an ISO accreditation
Core competencies acquired around the analysis of complex biological samples, notably in a clinical environment (diagnostic markers, therapeutic targets, marker validation, cohort studies, technological developments)
A wide range of cutting-edge equipment available (see list below)
Laboratory Infrastructure
The platform boasts an impressive infrastructure (over 20 specialist laboratory machines and applications) including:
MALDI TOF TOF mass spectrometer (Abi 4800)
Electrospray (4000 QTrap)
1 and 2D Nanochromatographers
Multi dimensional HPLC (Dionex)
Low pression chromatography system
At a glance: Examples of past/current projects
Identification of proteins demonstrating pharmacological activity originating from snake venom
Identification of proteins secreted by pulmonary tumor cell lines
Characterisation of synthetic peptides demonstrating pharmacological activity
Proteome stem cell research within the context of reparative vascular therapy
Research into biomarkers in plasma for chronic sleep apnea
Validation of tryptic digestion bioreactor in complex biological sample
Potential Applications
Protein identification in complex biological samples
Post translational modification studies
Protein Characterisation
Biomarker detection
Protein mapping
Quantative and differential analysis
The platform can use the iTRAQ technique to identify and quantify proteins from different sources in one single experiment. iTRAQ is used in proteomics to study quantitative changes in the proteome.
Identification of specific biomarkers
Analysis of the effects of drugs
Investigation of pathological mechanisms
Cinetic analysis
Grenoble Proteomic/Transcriptomic Platform
Manager : Issartel Jean-Paul / F Berger DR CNRS Grenoble Institut des Neurosciences
Equipment Proteomic analysis • SELDI TOF mass spectrometer - ProteinChip SELDI System Enterprise Edition (and associated software) • Biomek Robot used to prepare SELDI TOF protein chips • Bio-Rad BioPlex Suspension array system
Scientific analysis of nucleic acids (DNA, RNA, Non coding RNA) • Robots Spotter • Hybridisation oven • FUJI Phosphorimager • Agilent BioAnalyzer • Affymetrix WorkStation (et associated software)
Description of technical competencies • Preparation of RNA and QC (RNA and microRNA) • Transcriptomic analyses on Affymetrix micro-arrays • Analyses of complex proteins in biological samples by SELDI TOF (surface enhanced laser desorption ionization time of flight) mass spectrometry • Research into discriminating markers for biological or pathological phenotypes • Pre-treatment of samples for detection of proteins present in small quantities (Proteominer protein enrichment) • Multiplex analyses of proteins (color-coded beads sets xMAP technology)
Focus of themes targeted by the department Cancer (brain, bladder, leukemia) general or degenerative pathologies (sleep apnea, Parkinson, rheumatoid arthritis), parisites, immunology, oxidative stress, angiogenesis)
Current developments, installation of new technologies • MicroRNA analyses by hybridisation • Protein microdosages with micro-biopsy tools • Genotyping on the Affymetrix platform
Contact Carole Speziani 04 56 00 60 43
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